Reagents and Equipment

Plant Materials:

• brown coconuts
• lemons
• oranges
• limes
• grapefruit
• kiwis
• ginger

Equipment and facilities available

(in addition to that normally available in the molecular biosciences teaching lab)

• hammers and polythene bags to smash open coconuts
• coconut borer
• chopping boards
• knives
• graters
• juicers
• zesters
• peelers
• blenders
• sieves
• duran bottles
• 15 ml glass bottles
• beakers
• conical flasks
• cylinders
• funnels
• pestles and mortars
• sand for grinding samples
• muslin
• metal tweezers
• plastic food bags
• ethanol baths & spreaders
• vacuum centrifuge
• stir plates and magnetic followers
• freezer boxes
• autoclave (will be operated for you)
• 37 ^oC incubation facilities (non-shaking)
• 4 ^oC storage facilities
• 50 ^oC molten agar storage/plate warming incubator
• Consumables 10 mm Disk diffusion discs (sterile ones provided in the first
• session, non-sterile thereafter)
• Petri dishes (manufacturer supplies as sterile)
• 15 ml and 50 ml falcon tubes (manufacturer supplies as
• sterile)
• 25 ml universal tubes (manufacturer supplies as
• sterile)
• 7 ml bijou tubes (manufacturer supplies as sterile)
• 1.5 ml and 0.5 ml eppendorfs (sterilised)
• 1 ml cuvettes
• Round filter paper
• Cling film
• Foil

Reagents

• LB powder (makes liquid medium)
• agar powder (add to LB to make solid medium)
• isopropanol
• acetone
• ethanol
• 500 ml prepared molten LB agar per group for the first session only
• 100 ml prepared molten LB top agar per group for the first session only

Bacterial Strains

(provided as 5 ml liquid cultures grown overnight in LB)

• Escherichia coli
• Staphylococcus epidermidis

Antibiotics

kanamycin (50 mg/ml)