Technical information
Elizabeth Alvey; Emily Bailes; Janet Cronshaw; and Melanie Stapleton
Based on ~40 groups, provide the following on each of the three experimental days (amounts based on ~40 groups):
- LB agar plates: ~2000-3000 needed for around 40 groups over the three days
- 2 ml S. marcescens: enough for 1 per group per session. Culture at around 1 x 10-9 cfu/ml (i.e. an overnight culture grown aerobically in LB at 200 rpm 37°C)
- Empty petri dishes: box
- Sterile 50 ml falcons (suitable for centrifuges): box
- Sterile 15 ml falcons: box
- Sterile 7 ml bijous: box
- Sterile 1.5 ml Eppendorfs: few boxes
- Sterile swabs: enough for around 50 per group
- Alcohol baths & spreaders: enough for 1 per group per session
- Ethanol: 2 x 2.5 L bottles
- Funnels: (small) box
- Tweezers: box
- 6-inch smooth white tiles: all that we have
- Apples: 30-50 (put out a few to start with)
- Cheese: 3-5 kg (put out a few to start with)
- Grapes: 4-8 x 500g packs (put out a few to start with)
- Sterile marbles in pots of ~10: enough for a pot per group per session
- Sterile wooden blocks in beakers of ~5: enough for a pot per group per session
- Chopping boards & knives: one per group
- Virkon pots: one per group (for contaminated labware, such as tweezers)
- LB broth in 1 L Durans: put 2 x 1 L per session in a flow hood with sterilised Oxford dispensers set to 5 ml. Students come and help themselves
- Sterile 20 ml universals: a box in/next to the flow hood containing LB dispensers
- Waste ethanol bottle & funnel
- Tubs of virkon several, on the sinks for contaminated items for washing e.g. tiles, marbles, wooden blocks
- Draining racks, brushes and washing up liquid: several, on the sinks (for them to do their own washing up – non-contaminated items only)
- Boxes for students’ plates – to be incubated overnight at 30°C
