Resources for teachers
Rebecca Barnes and Melanie Stapleton
Model results
PCR
| Lane | Plasmid | Primers |
| 1 | 100 bp GeneRuler | – |
| 2 | P2 | Amp-F & Amp-R |
| 3 | Kan-F & Kan-R | |
| 4 | P1 | Amp-F & Amp-R |
| 5 | Kan-F & Kan-R | |
| 6 | 1 kb GeneRuler | – |
Restriction digests
| Lane | DNA | Restriction enzyme(s) |
| 1 | 1 kb GeneRuler | – |
| 2 | P1 | – |
| 3 | EcoRV | |
| 4 | SmaI | |
| 5 | EcoRV + SmaI | |
| 6 | P2 | – |
| 7 | EcoRV | |
| 8 | SmaI | |
| 9 | EcoRV + SmaI | |
| 10 | 1 kb GeneRuler | – |
